Listening to the Night: Studying Bats Through Sound at Cloudbridge

At Cloudbridge, understanding the wildlife that inhabits the cloud forest is a fundamental part of protecting it. This month, we are excited to share a new research project focused on one of the reserve’s most elusive and fascinating groups of animals: bats.

Together with bat researcher Doris Audet, Cloudbridge has started a project aimed at identifying the bat species present in the reserve through acoustic monitoring, a technique that records and analyzes bat echolocation calls. But how does this process actually work in the field?

The first step is selecting the recording locations. Before going into the forest, different points are chosen on a map to make sure we cover a variety of habitats across the reserve, including different elevations, forest types, and levels of vegetation cover. This helps researchers better understand which bat species are using different parts of the cloud forest.

Field equipment used for acoustic bat monitoring at Cloudbridge, including the ultrasonic recorder, microphone, batteries, mounting materials, and field sheets used to document the details of each recording site.
The ultrasonic microphone mounted on a three-meter pole after installation at one of Cloudbridge’s monitoring sites.

Once in the field, the team carries the recording equipment to each selected site. At every location, a specialized ultrasonic microphone is mounted on a pole about three meters above the ground. Raising the microphone helps improve the quality of the recordings and allows it to better capture the calls of bats flying through the forest.

The microphone is connected to an acoustic recorder, which is carefully programmed before being left in place. The recorder is set to begin working shortly before sunset and continue recording until shortly after sunrise, when bats are most active. It is also adjusted to record only the ultrasonic frequencies used by bats, filtering out many other forest sounds.

The equipment then remains in the same location for two nights, recording continuously throughout the night. On the third day, the recorder is moved to another preselected site, allowing the project to sample many different areas of the reserve over time.

Close-up of the acoustic recorder and ultrasonic microphone after installation in the field.

One of the greatest advantages of this technique is that it is completely passive and non-invasive. The bats do not need to be captured, handled, or disturbed in any way. The detectors simply record the sounds naturally produced by bats as they move through the forest. Because the equipment records all night long, researchers can also collect much more information than during a short field survey. Acoustic monitoring is especially useful in cloud forests because it can detect bats flying at different levels of the forest, from the understory close to the ground to the upper canopy, areas that are often difficult to study using traditional methods.

However, collecting the recordings is only the beginning of the process. After the equipment is retrieved, the data must be carefully processed using specialized software that searches through the recordings for recognizable bat calls. The software can visualize the calls and measure characteristics such as their frequency, duration, and pattern.

This final step requires extensive experience and expert knowledge. Many bat species produce calls that can sound very similar, while environmental noise, rain, insects, or overlapping sounds can make analysis even more challenging. Specialists like Doris Audet are therefore essential to the project. Through her expertise, Doris can interpret the patterns and frequencies of the calls and determine which species produced them.

Bat echolocation recordings visualized on a computer during the analysis process. In this example, the graph shows both search calls, with frequencies around 59–60 kHz, and a feeding buzz, visible as a rapid increase in call rate accompanied by a drop in frequency. These calls belong to a bat from the genus Myotis (Family Vespertilionidae), likely Myotis pilosatibialis, one of the most frequently recorded bat call types at Cloudbridge.

So far, the results have already been very exciting. Bat activity was detected across all forest types, elevations, and monitoring sites, with recordings indicating the presence of at least eight species from three different bat families.

Some of the most commonly recorded bats belong to the genus Myotis, including Myotis pilosatibialis, Myotis armiensis, Myotis nigricans, and possibly Myotis oxyotus, a highland species that had not previously been recorded at Cloudbridge during past bat counts. Free-tailed bats, including Tadarida brasiliensis and at least one species from the genus Molossus, were also frequently detected.

Among the most exciting discoveries so far were two uncommon sac-winged bats from the family Emballonuridae: Centronycteris centralis (the Shaggy Bat) and Peropteryx kappleri (the Greater Dog-like Bat). Both species were detected only at single sites within older-growth forest areas, making these especially remarkable findings for the project.

These are only the first results of the analysis, and there is still a large amount of data left to process. As more recordings are reviewed, the project may reveal additional species and provide even more valuable information about the bat diversity of Cloudbridge.

Projects like this are extremely valuable for Cloudbridge. Every new piece of information helps us better understand the biodiversity of the cloud forest and the species that depend on it, helping guide future conservation efforts. Cloudbridge is always excited to welcome collaborative research projects that deepen our understanding of tropical cloud forests and the incredible wildlife that calls them home.

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